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KMID : 0368419920350010037
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Journal of Plant Biology
1992 Volume.35 No. 1 p.37 ~ p.43
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Production of Anthocyanin by Culture of Hairy Roots of Raphanus sativus cv. Chungpihongsim
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安俊徹/Ahn, Jun Cheul
白允雄/康榮熹/황백/Paek, Yun Woung/Kang, Young Hee/Hwang, Baik
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Abstract
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The hairy root culture of Raphanus sativus cv. Chungpihongsim was established by transformation with Agrobacterium rhizogenes A_4. The transformed roots grew well in adjusted Murashige and Skoog medium to 1/2 basal salts, pH5.2,3% sucrose. Agropine and mannopine, poine synthesized in the transformed tissue were detected in the extract of hairy roots. When 2,4-D and kinetin were added in culture medium of hairy roots, the synthesis of anthocyanin was induced with disorganization of hairy root. Especially, addition of 0.45 μM 2,4-D and 2.3 μM kinetin showed the maximum synthesis of anthocyanin. Pattern of anthocyanin synthesized in transformed roots was somewhat different from that of ordinary roots. However, aglycone part of all anthocyanin was identified as pelargonidin. The content of total anthocyanin in this sample was tentatively calculated 0.49 mg/g fresh weight.
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